Taq DNA Polymerase is a 94 kD thermostable enzyme. Its optimum temperature of activity is between 55? C and 75? C. The enzyme lacks 3′ to 5′ exonuclease (proof reading) activity but has an inherent 5′ to 3′ exonuclease activity.

Specification :
The enzyme is supplied at concentrations of 1 U/μl, 3 U/μl, 5 U/μl.
Taq DNA Polymerase Buffers : Refer the chart for buffer composition.
Buffer supplied at 10X concentration.

Storage and Dilution buffer :
Tris-HCl (pH 8), KCl, EDTA, DTT, Tween 20, Igepal and Glycerol.

Storage : -20°C

Application :
• Amplification of DNA fragments of varied sizes ranging from about 100 bp to 3kb by polymerase chain reaction.
•The enzyme can be used in RAPD studies to detect polymorphism in several species.
• Taq DNA Polymerase can be supplied with :
• 10X buffer containing 15 mM MgCl₂ or
• 10X buffer and separate vial of 25 mM MgCl₂

Note : Concentration of the components of the buffer are proprietary.

Performance Test :
1. Taq DNA Polymerase is extensively tested for its performance in several PCR Amplifications, RAPD, RT-PCR.
2. Taq DNA Polymerase is also tested in nested PCR using WSSV detection kit

Storage : -20°C