Total Dietary Fiber Assay Kit

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Total Dietary Fiber Assay Kit

Category: Mobile & Photometry
Brand: Sigma
Catalog Number: TDF100A
GST: 12%
HSN Code: 38220090
Pack: 1 kit
Hazardous: No
EUD: Not applicable

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Description

Total Dietary Fiber Assay Kit

Application

This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.

Packaging

1 kit

Other Notes

Reagents for the determination of total dietary fiber.

Principle

Uses a combination of enzymatic and gravimetric methods. This procedure is based on the method published in Official Methods of Analysis, 15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).

Kit Components Only

Product No.
Description
α-Amylase, Heat-stable 10 mL
Amyloglucosidase 10 mL
Celite 50 g
Protease 500 mg

PROPERTIES

usage

sufficient for ~100 assays

Quality Level

100

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical