TRIZMA(R) BASE, BIOPERFORMANCE CERTIF&

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TRIZMA(R) BASE, BIOPERFORMANCE CERTIF&

Category: Biochemicals & Reagents
Brand: Sigma
Catalog Number: T6066-5KG
GST: 18%
HSN Code: 29221990
Pack: 1 EA
Hazardous: No
EUD: Not applicable

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Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma has been used:

As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
As a component of pre-hybridization buffer in in situ hybridization
To suspend dried protein before SDS-PAGE protein characterization
As a component of lysis buffer to wash and lyse cells before immunoprecipitation
In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

5 kg in poly drum

T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Easily compare specifications for Trizma products with the Trizma specification table.